DNA chips have become popular tools for gene discovery and also for diagnostics. A genome-wide DNA microarray for cassava with ~ 14,000 probes has been developed. The microarray has been utilized for transcriptome analysis of cassava (Figure 2). Candidate genes that are differentially expressed after virus infection have been identified. In addition to gene discovery, DNA chips provide a reverse genetics tool for identification of gene-targeted markers for molecular breeding. A genome-wide DNA microarray that is specific for cassava has been developed. This is the most comprehensive DNA chip for cassava available to date.
Application of Diversity Array Technology (DArT) markers in cassava genotyping
A cassava DArT chip with 735 polymorphic markers was used to fingerprint a diverse cassava population comprising genotypes from Africa, Latin America, Asia and breeder lines maintained at the International Institute of Tropical Agriculture. Overall reproducibility of the marker set was very high and average call rate was 97%. DArT markers provide reliable and high throughput molecular information for the management of biodiversity in germplasm collections and enables rapid genome profiles for QTL mapping.
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